Genetic Polymorphisms of The Chicken Antiviral Mx Gene in A Variety of Indonesian Indigenous Chicken Breeds

Genetic Polymorphisms of The Chicken Antiviral Mx Gene in A Variety of Indonesian Indigenous Chicken Breeds

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It has previously been demonstrated that a G/A Single Nucleotide Polymorphism (SNP) at nucleotideposition 1,892 of coding sequence of chicken Mx gene confers susceptibility/resistance to avian viral diseases.The aim of this study was to assess the geographical distribution earthbath facial wipes of G/A alleles in relation to differentgenetic backgrounds of a wide range of chicken populations.Using Polymerase Chain Reaction- RestrictionFragment Length Polymorphism (PCR-RFLP) methods, 492 samples from 15 breeds of indigenous chickenpopulations from Java, Sumatera, Kalimantan and Sulawesi tillman 750m islands were genotyped.

Allele and genotypefrequencies of each population were calculated.Deviations from Hardy-Weinberg equilibrium were testedand inbreeding coefficient FIS estimated.Overall, the susceptible allele G had a frequency of 37.

27% whilethe resistant allele A had a corresponding frequency of 62.73%.No clear relation of the geographicaldistribution of the G/A alleles to genetic backgrounds was found.

The distribution of this SNP acrosspopulations seems to be affected by genetic drift rather than selection.